Posts Tagged: CRISPR

An alternative TALEN/CRISPR-mediated gene insertion technique described in detail

An alternative TALEN/CRISPR-mediated gene insertion technique described in detail

  A streamlined protocol for an alternative gene insertion method using genome editing technologies, the PITCh (Precise Integration into Target Chromosome) system, has been reported in┬áNature Protocols┬áby Specially Appointed Lecturer Tetsushi Sakuma, Professor Takashi Yamamoto, Specially Appointed Associate Professor Ken-Ichi T Suzuki, and their colleagues at Hiroshima University, Japan. The PITCh system is more convenient… Read more

Simultaneous live imaging of a specific gene’s transcription and dynamics

Simultaneous live imaging of a specific gene’s transcription and dynamics

The Real-time Observation of Localization and EXpression (ROLEX) system   Dr. Hiroshi Ochiai and his colleagues, Dr. Takeshi Sugawara (Research Center for the Mathematics on Chromatin Live Dynamics [RcMcD] at Hiroshima University) and Professor Takashi Yamamoto (Graduate School of Science at Hiroshima University), have established a novel live-imaging method termed the “Real-time Observation of Localization… Read more

A novel technique for gene insertion by genome editing

A novel technique for gene insertion by genome editing

Easy, accurate, and highly efficient gene knock-in in a variety of cells and organisms Using a novel gene knock-in technique, effective insertion of an exogenous gene was demonstrated in human cells and in animal models, including silkworms and frogs. This strategy universally enables gene knock-in not only in cultured cells, but also in various organisms…. Read more